Alternative Names:
TPBG; 5T4; 5T4-AG; 5T4AG; 5T4 oncofetal antigen; M6P1; Trophoblast glycoprotein;
Application:
WB IHC
Reactivity:
Human
Dilution:
WB 1:500~1:1000 IHC 1:50~1:200
 Western blot analysis of 5T4 expression in Caco-2 cell lysates. |
 Immunohistochemical analysis of paraffin-embedded human uters cancer, using 5T4 Antibody. |
Purification:
Affinity-chromatography
Specificity:
5T4 Antibody detects endogenous levels of 5T4
Immunogen:
A synthesized peptide derived from human 5T4
Description:
5T4 is a 72 kDa transmembrane glycoprotein that exhibits restricted expression in human and mouse adult tissues, is upregulated on many carcinomas and tumour expression correlates with poorer clinical outcome in ovarian, gastric and colorectal cancers. mES cells lack cell surface expression of the 5T4 antigen, and both protein and mRNA are rapidly upregulated following induction of differentiation. This proteins expression correlates with downregulation of OCT-4 and Tra-1–60.
Storage Condition and Buffer:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
