GFP ; yfp ; Green fluorescent protein ;
|Western blot analysis of GFP fusion protein expression in (1) 293T cell lysate; (2) 293T cell transfected GFP fusion protein with GFP Antibody(HRP conjugated).|
GFP Antibody(HRP conjugated) detects endogenous levels of C-terminal, internal, and N-terminal GFP-tagged proteins.
A synthesized peptide
Green fluorescent protein (GFP) has been engineered to produce a vast number of variously colored mutants, fusion proteins, and biosensors. Fluorescent proteins and its mutated allelic forms, blue, cyan and yellow have become a useful and ubiquitous tool for making chimeric proteins, where they function as a fluorescent protein tag. Typically they tolerate N- and C-terminal fusion to a broad variety of proteins.
Storage Condition and Buffer:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.